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Leica, Jena, Germany)

Briefly, an aliquot of cell homogenate was incubated with the labeled substrate DEVD-pNA (acetyl-Asp-Glu-Val-Asp p-nitroaniline), which is cleaved by the caspase-3 enzyme and releases the chromophore pNA

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A damaged skin barrier can perpetuate inflammation and oxidative stress, both of which trigger increased melanin production as a protective response